• Immunofluorescence analysis of MCF7 cells using RPL14 antibody (STJ28807). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using RPL14 antibody (STJ28807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RPL14 antibody (1-215) (STJ28807)

SKU:
STJ28807

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPL14 (1-215) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPL14
Gene ID: 9045
Uniprot ID: RL14_HUMAN
Immunogen Region: 1-215
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RPL14 (NP_003964.3).
Immunogen Sequence: MVFRRFVEVGRVAYVSFGPH AGKLVAIVDVIDQNRALVDG PCTQVRRQAMPFKCMQLTDF ILKFPHSAHQKYVRQAWQKA DINTKWAATRWAKKIEARER KAKMTDFDRFKVMKAKKMRN RIIKNEVKKLQKAALLKASP KKAPGTKGTAAAAAAAAAAK VPAKKITAASKKAPAQKVPA QKATGQKAAPAPKAQKGQKA PAQKAPAPKASGKKA
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protein Name Large Ribosomal Subunit Protein El14
60s Ribosomal Protein L14
Cag-Isl 7
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein El14 antibody
Anti-60s Ribosomal Protein L14 antibody
Anti-Cag-Isl 7 antibody
Anti-RPL14 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance