• Western blot analysis of extracts of various cell lines, using RPIA antibody (STJ111456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of RPIA in paraffin-embedded rat kidney using RPIA Rabbit polyclonal antibody (STJ111456) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat heart using RPIA antibody (STJ111456) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of RPIA in paraffin-embedded rat heart using RPIA Rabbit polyclonal antibody (STJ111456) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat kidney using RPIA antibody (STJ111456) at dilution of 1:100 (40x lens).
  • Western blot analysis of various lysates using RPIA Rabbit polyclonal antibody (STJ111456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-RPIA antibody (82-311) (STJ111456)

SKU:
STJ111456

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPIA (82-311) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPIA
Gene ID: 22934
Uniprot ID: RPIA_HUMAN
Immunogen Region: 82-311
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 82-311 of human RPIA (NP_653164.2).
Immunogen Sequence: KKLAGRAAVENHVRNNQVLG IGSGSTIVHAVQRIAERVKQ ENLNLVCIPTSFQARQLILQ YGLTLSDLDRHPEIDLAIDG ADEVDADLNLIKGGGGCLTQ EKIVAGYASRFIVIADFRKD SKNLGDQWHKGIPIEVIPMA YVPVSRAVSQKFGGVVELRM AVNKAGPVVTDNGNFILDWK FDRVHKWSEVNTAIKMIPGV VDTGLFINMAERVYFGMQDG SVNMREKPFC
Function Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate and participates in the first step of the non-oxidative branch of the pentose phosphate pathway.
Protein Name Ribose-5-Phosphate Isomerase
Phosphoriboisomerase
Database Links Reactome: R-HSA-5659996
Reactome: R-HSA-6791461
Reactome: R-HSA-71336
Alternative Antibody Names Anti-Ribose-5-Phosphate Isomerase antibody
Anti-Phosphoriboisomerase antibody
Anti-RPIA antibody
Anti-RPI antibody

Information sourced from Uniprot.org

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