• Western blot analysis of HeLa, using RPF2 antibody (STJ119350) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RPF2 antibody (1-306) (STJ119350)

SKU:
STJ119350

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPF2 (1-306) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPF2
Gene ID: 84154
Uniprot ID: RPF2_HUMAN
Immunogen Region: 1-306
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of human RPF2 (NP_115570.1).
Immunogen Sequence: MDTLDRVVKPKTKRAKRFLE KREPKLNENIKNAMLIKGGN ANATVTKVLKDVYALKKPYG VLYKKKNITRPFEDQTSLEF FSKKSDCSLFMFGSHNKKRP NNLVIGRMYDYHVLDMIELG IENFVSLKDIKNSKCPEGTK PMLIFAGDDFDVTEDYRRLK SLLIDFFRGPTVSNIRLAGL EYVLHFTALNGKIYFRSYKL LLKKSGCRTPRIELEEMGPS LDLVLRRTHLASDDLYKLS
Function Involved in ribosomal large subunit assembly. May regulate the localization of the 5S RNP/5S ribonucleoprotein particle to the nucleolus.
Protein Name Ribosome Production Factor 2 Homolog
Brix Domain-Containing Protein 1
Ribosome Biogenesis Protein Rpf2 Homolog
Cellular Localisation Nucleus
Nucleolus
Associated With The Nucleolus In An Rna-Dependent Manner
Alternative Antibody Names Anti-Ribosome Production Factor 2 Homolog antibody
Anti-Brix Domain-Containing Protein 1 antibody
Anti-Ribosome Biogenesis Protein Rpf2 Homolog antibody
Anti-RPF2 antibody
Anti-BXDC1 antibody

Information sourced from Uniprot.org

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