Anti-RPE65 antibody (STJA0006983)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human RPE65
General Information
| Short Description | Rabbit polyclonal anti-Retinoid isomerohydrolase for use in WB in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RPE65 |
| Gene ID | 6121 |
| Uniprot ID | RPE65_HUMAN |
| Immunogen | Synthesized peptide derived from human RPE65 |
| Specificity | This antibody detects endogenous levels of RPE65 at Human, Rat |
Additional Info
| Post Translational Modifications | Palmitoylation by LRAT regulates ligand binding specificity.the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A). |
| Function | Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. |
| Protein Name | Retinoid IsomerohydrolaseAll-Trans-Retinyl-Palmitate HydrolaseLutein IsomeraseMeso-Zeaxanthin IsomeraseRetinal Pigment Epithelium-Specific 65 Kda ProteinRetinol Isomerase |
| Database Links | Reactome: R-HSA-2453902 |
| Cellular Localisation | CytoplasmCell MembraneLipid-AnchorMicrosome MembraneAttached To The Membrane By A Lipid Anchor When Palmitoylated (Membrane Form)Soluble When UnpalmitoylatedUndergoes Light-Dependent Intracellular Transport To Become More Concentrated In The Central Region Of The Retina Pigment Epithelium Cells |
| Alternative Antibody Names | Anti-Retinoid Isomerohydrolase antibodyAnti-All-Trans-Retinyl-Palmitate Hydrolase antibodyAnti-Lutein Isomerase antibodyAnti-Meso-Zeaxanthin Isomerase antibodyAnti-Retinal Pigment Epithelium-Specific 65 Kda Protein antibodyAnti-Retinol Isomerase antibodyAnti-RPE65 antibody |
Information sourced from Uniprot.org