Anti-RPE65 antibody (STJ502831)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide corresponding to unique amino acid sequence on mouse RPE65 protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-RPE65 is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IP/WB |
| Host | Rabbit |
| Reactivity | Bovine/Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.51-0.63 µg/µl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:500 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Rpe65 |
| Gene ID | 19892 |
| Uniprot ID | RPE65_MOUSE |
| Immunogen | Synthetic peptide corresponding to unique amino acid sequence on mouse RPE65 protein. |
Additional Info
| Tissue Specificity | Retinal pigment epithelium specific. |
| Post Translational Modifications | Palmitoylation by LRAT regulates ligand binding specificity.the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A). |
| Function | Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. |
| Protein Name | Retinoid IsomerohydrolaseAll-Trans-Retinyl-Palmitate HydrolaseLutein IsomeraseMeso-Zeaxanthin IsomeraseRetinal Pigment Epithelium-Specific 65 Kda ProteinRetinol Isomerase |
| Database Links | Reactome: R-MMU-2453902 |
| Cellular Localisation | CytoplasmCell MembraneLipid-AnchorMicrosome MembraneAttached To The Membrane By A Lipid Anchor When Palmitoylated (Membrane Form)Soluble When UnpalmitoylatedUndergoes Light-Dependent Intracellular Transport To Become More Concentrated In The Central Region Of The Retina Pigment Epithelium Cells |
| Alternative Antibody Names | Anti-Retinoid Isomerohydrolase antibodyAnti-All-Trans-Retinyl-Palmitate Hydrolase antibodyAnti-Lutein Isomerase antibodyAnti-Meso-Zeaxanthin Isomerase antibodyAnti-Retinal Pigment Epithelium-Specific 65 Kda Protein antibodyAnti-Retinol Isomerase antibodyAnti-Rpe65 antibody |
Information sourced from Uniprot.org