Anti-RPAIN antibody (117-167 aa) (STJ195501)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human RIP at the amino acid range 117-167
General Information
| Short Description | Rabbit polyclonal anti-RPA-interacting protein (117-167 aa) for use in WB, ELISA and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RPAIN |
| Gene ID | 84268 |
| Uniprot ID | RIP_HUMAN |
| Immunogen | Synthesized peptide derived from the human RIP at the amino acid range 117-167 |
| Immunogen Region | 117-167 aa |
| Specificity | This antibody detects endogenous levels of RIP at Human/Mouse/Rat |
Additional Info
| Post Translational Modifications | Isoform 2: Sumoylated. Sumoylation is required for localization in the nuclear PML body and transport of RPA complex in PML body. Upon UV irradiation and during S phase, it is desumoylated, releasing RPA complex that is translocated to sites of DNA damage. Sumoylation takes place at different Lys residues. Variant 'Lys-103' adds a sumoylation site and increases total sumoylation levels. |
| Function | Mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. Isoform 2 is sumoylated and mediates the localization of RPA complex into the PML body of the nucleus, thereby participating in RPA function in DNA metabolism. |
| Protein Name | Rpa-Interacting ProteinHrip |
| Database Links | |
| Cellular Localisation | Isoform 1: CytoplasmNucleusIsoform 2: NucleusPml Body |
| Alternative Antibody Names | Anti-Rpa-Interacting Protein antibodyAnti-Hrip antibodyAnti-RPAIN antibodyAnti-RIP antibody |
Information sourced from Uniprot.org