• Immunohistochemistry analysis of paraffin-embedded mouse retina using ROM1 antibody (STJ11100930) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ROM1 antibody (STJ11100930) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ROM1 antibody (126-263) (STJ11100930)

SKU:
STJ11100930

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ROM1 (126-263) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ROM1
Gene ID: 6094
Uniprot ID: ROM1_HUMAN
Immunogen Region: 126-263
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 126-263 of human ROM1 (NP_000318.1).
Immunogen Sequence: PGSLDEALEEGLVTALAHYK DTEVPGHCQAKRLVDELQLR YHCCGRHGYKDWFGVQWVSS RYLDPGDRDVADRIQSNVEG LYLTDGVPFSCCNPHSPRPC LQNRLSDSYAHPLFDPRQPN QNLWAQGCHEVLLEHLQD
Tissue Specificity Retina photoreceptors (at protein level). In rim region of ROS disks.
Function Plays a role in rod outer segment (ROS) morphogenesis. May play a role with PRPH2 in the maintenance of the structure of ROS curved disks. Plays a role in the organization of the ROS and maintenance of ROS disk diameter. Involved in the maintenance of the retina outer nuclear layer.
Protein Name Rod Outer Segment Membrane Protein 1
Rosp1
Tetraspanin-23
Tspan-23
Cellular Localisation Photoreceptor Inner Segment Membrane
Multi-Pass Membrane Protein
Photoreceptor Outer Segment Membrane
Alternative Antibody Names Anti-Rod Outer Segment Membrane Protein 1 antibody
Anti-Rosp1 antibody
Anti-Tetraspanin-23 antibody
Anti-Tspan-23 antibody
Anti-ROM1 antibody
Anti-TSPAN23 antibody

Information sourced from Uniprot.org

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