• Western blot analysis of extracts of various cell lines, using ROBO4 antibody (STJ111918) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using ROBO4 antibody (STJ111918) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ROBO4 antibody (848-1007) (STJ111918)

SKU:
STJ111918

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ROBO4 (848-1007) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ROBO4
Gene ID: 54538
Uniprot ID: ROBO4_HUMAN
Immunogen Region: 848-1007
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 848-1007 of human ROBO4 (NP_061928.4).
Immunogen Sequence: GVGPKGGVLLCPPRPCLTPT PSEGSLANGWGSASEDNAAS ARASLVSSSDGSFLADAHFA RALAVAVDSFGFGLEPREAD CVFIDASSPPSPRDEIFLTP NLSLPLWEWRPDWLEDMEVS HTQRLGRGMPPWPPDSQISS QRSQLHCRMPKAGASPVDYS
Tissue Specificity Specifically expressed in endothelial cells. Expressed in endothelial and intimal cells of the ascending aorta.
Function Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Involved in the maintenance of endothelial barrier organization and function.
Protein Name Roundabout Homolog 4
Magic Roundabout
Alternative Antibody Names Anti-Roundabout Homolog 4 antibody
Anti-Magic Roundabout antibody
Anti-ROBO4 antibody
Anti-UNQ421 antibody
Anti-PRO3674 antibody

Information sourced from Uniprot.org

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