Anti-Robo3 antibody (C-Term) (STJ13101948)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide to from c-terminal region of mouse Robo3 (Roundabout homolog 3) conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-Robo3 (C-Term) for use in IHC and WB in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Robo3 |
| Gene ID | 19649 |
| Uniprot ID | ROBO3_MOUSE |
| Immunogen | A synthetic peptide to from c-terminal region of mouse Robo3 (Roundabout homolog 3) conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | C-Term |
| Specificity | Specific for Robo3. |
Additional Info
| Function | Receptor involved in axon guidance during development. Acts as a multifunctional regulator of pathfinding that simultaneously mediates NELL2 repulsion, inhibits SLIT repulsion, and facilitates Netrin-1/NTN1 attraction. In spinal cord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1/ROBO2. Binding OF NELL2 to the receptor ROBO3 promotes oligomerization of ROBO3, resulting in the repulsion of commissural axons in the midline. ROBO3 also indirectly boosts axon attraction to NTN1 without interacting with NTN1 itself. Isoform 1: Mediates NELL2 premature repulsion of commissural axons during midline crossing. Isoform 2: After midline crossing by the commissural axons, may, in concert with ROBO1 and ROBO2, prevent midline recrossing. Does not mediate NELL2 signaling. |
| Protein Name | Roundabout Homolog 3Retinoblastoma-Inhibiting Gene 1 ProteinRig-1Roundabout Guidance Receptor 3 |
| Database Links | |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Roundabout Homolog 3 antibodyAnti-Retinoblastoma-Inhibiting Gene 1 Protein antibodyAnti-Rig-1 antibodyAnti-Roundabout Guidance Receptor 3 antibodyAnti-Robo3 antibodyAnti-Rbig1 antibody |
Information sourced from Uniprot.org