| Short Description : | Rabbit polyclonal RNGTT (1-260) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RNGTT |
| Gene ID: | 8732 |
| Uniprot ID: | MCE1_HUMAN |
| Immunogen Region: | 1-260 |
| Immunogen Sequence: | MAHNKIPPRWLNCPRRGQPV AGRFLPLKTMLGPRYDSQVA EENRFHPSMLSNYLKSLKVK MGLLVDLTNTSRFYDRNDIE KEGIKYIKLQCKGHGECPTT ENTETFIRLCERFNERNPPE LIGVHCTHGFNRTGFLICAF LVEKMDWSIEAAVATFAQAR PPGIYKGDYLKELFRRYGDI EEAPPPPLLPDWCFEDDEDE DEDEDGKKESEPGSSASFGK RRKERLKLGAIFLEGVTVK |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RNGTT (NP_003791.3). |
| Tissue Specificity | Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle. |
| Function | Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphate monophosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the GMP moiety of GTP to the 5'-diphosphate terminus of RNA via a covalent enzyme-GMP reaction intermediate. |
| Protein Name | Mrna-Capping EnzymeHcap1Hce Includes - Mrna 5'-Triphosphate MonophosphataseMrna 5'-Phosphatase - Mrna GuanylyltransferaseGtp--Rna GuanylyltransferaseGtase |
| Database Links | Reactome: R-HSA-167160Reactome: R-HSA-72086Reactome: R-HSA-77075 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Mrna-Capping Enzyme antibodyAnti-Hcap1 antibodyAnti-Hce Includes - Mrna 5'-Triphosphate Monophosphatase antibodyAnti-Mrna 5'-Phosphatase - Mrna Guanylyltransferase antibodyAnti-Gtp--Rna Guanylyltransferase antibodyAnti-Gtase antibodyAnti-RNGTT antibodyAnti-CAP1A antibody |
Information sourced from Uniprot.org
