• Western blot analysis of extracts of various cell lines, using RNF40 rabbit monoclonal antibody (STJ11102500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using RNF40 Rabbit monoclonal antibody (STJ11102500) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of mouse brain, using RNF40 rabbit monoclonal antibody (STJ11102500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of Mouse brain, using RNF40 Rabbit monoclonal antibody (STJ11102500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using RNF40 rabbit monoclonal antibody (STJ11102500) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using RNF40 Rabbit monoclonal antibody (STJ11102500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RNF40 antibody (231-431) [S0MR] (STJ11102500)

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STJ11102500

$197.78 - $863.33
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RNF40 (231-431) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF40
Gene ID: 9810
Uniprot ID: BRE1B_HUMAN
Immunogen Region: 231-431
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 231-431 of human RNF40 (O75150).
Immunogen Sequence: RELGRENRRLQDLATQLQEK HHRISLEYSELQDKVTSAET KVLEMETTVEDLQWDIEKLR KREQKLNKHLAEALEQLNSG YYVSGSSSGFQGGQITLSMQ KFEMLNAELEENQELANSRM AELEKLQAELQGAVRTNERL KVALRSLPEEVVRETGEYRM LQAQFSLLYNESLQVKTQLD EARGLLLATKNSHLRHIEHM E
Tissue Specificity Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.
Function Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B.reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.
Protein Name E3 Ubiquitin-Protein Ligase Bre1b
Bre1-B
95 Kda Retinoblastoma-Associated Protein
Rbp95
Ring Finger Protein 40
Ring-Type E3 Ubiquitin Transferase Bre1b
Database Links Reactome: R-HSA-8866654
Cellular Localisation Nucleus
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Bre1b antibody
Anti-Bre1-B antibody
Anti-95 Kda Retinoblastoma-Associated Protein antibody
Anti-Rbp95 antibody
Anti-Ring Finger Protein 40 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Bre1b antibody
Anti-RNF40 antibody
Anti-BRE1B antibody
Anti-KIAA0661 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance