| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RNF4 (1-190) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF4 |
| Gene ID: |
6047 |
| Uniprot ID: |
RNF4_HUMAN |
| Immunogen Region: |
1-190 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RNF4 (NP_001171938.1). |
| Immunogen Sequence: |
MSTRKRRGGAINSRQAQKRT REATSTPEISLEAEPIELVE TAGDEIVDLTCESLEPVVVD LTHNDSVVIVDERRRPRRNA RRLPQDHADSCVVSSDDEEL SRDRDVYVTTHTPRNARDEG ATGLRPSGTVSCPICMDGYS EIVQNGRLIVSTECGHVFCS QCLRDSLKNANTCPTCRKKI NHKRYHPIYI |
| Tissue Specificity | Widely expressed at low levels in many tissues.highly expressed in testis. |
| Post Translational Modifications | Sumoylated.conjugated by one or two SUMO1 moieties. Autoubiquitinated. |
| Function | E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation. Catalyzes ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to PARP1 removal from chromatin by VCP/p97. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf4Ring Finger Protein 4Small Nuclear Ring Finger ProteinProtein Snurf |
| Database Links | Reactome: R-HSA-5693607Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusPml Body |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf4 antibodyAnti-Ring Finger Protein 4 antibodyAnti-Small Nuclear Ring Finger Protein antibodyAnti-Protein Snurf antibodyAnti-RNF4 antibodyAnti-SNURF antibodyAnti-RES4-26 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
