| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RNF34 (1-220) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF34 |
| Gene ID: |
80196 |
| Uniprot ID: |
RNF34_HUMAN |
| Immunogen Region: |
1-220 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human RNF34 (NP_079402.2). |
| Immunogen Sequence: |
MKAGATSMWASCCGLLNEVM GTGAVRGQQSAFAGATGPFR FTPNPEFSTYPPAATEGPNI VCKACGLSFSVFRKKHVCCD CKKDFCSVCSVLQENLRRCS TCHLLQETAFQRPQLMRLKV KDLRQYLILRNIPIDTCREK EDLVDLVLCHHGLGSEDDMD TSSLNSSRSQTSSFFTRSFF SNYTAPSATMSSFQGELMDG DQTSRSGVPAQVQSEITSAN |
| Tissue Specificity | Ubiquitous. Detected in heart, brain, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, colon and leukocytes. |
| Post Translational Modifications | Autoubiquitinated (in vitro). Proteolytically cleaved by caspases upon induction of apoptosis by TNF. |
| Function | E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells. Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf34Caspase Regulator Carp1Caspases-8 And -10-Associated Ring Finger Protein 1Carp-1Fyve-Ring Finger Protein MomoHuman Ring Finger Homologous To Inhibitor Of Apoptosis ProteinHrfiRing Finger Protein 34Ring Finger Protein RiffRing-Type E3 Ubiquitin Transferase Rnf34 |
| Database Links | Reactome: R-HSA-6804757Reactome: R-HSA-983168 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinEndomembrane SystemNucleusNucleus SpeckleCytoplasmCytosol |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf34 antibodyAnti-Caspase Regulator Carp1 antibodyAnti-Caspases-8 And -10-Associated Ring Finger Protein 1 antibodyAnti-Carp-1 antibodyAnti-Fyve-Ring Finger Protein Momo antibodyAnti-Human Ring Finger Homologous To Inhibitor Of Apoptosis Protein antibodyAnti-Hrfi antibodyAnti-Ring Finger Protein 34 antibodyAnti-Ring Finger Protein Riff antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf34 antibodyAnti-RNF34 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
