| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RNF20 (1-260) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF20 |
| Gene ID: |
56254 |
| Uniprot ID: |
BRE1A_HUMAN |
| Immunogen Region: |
1-260 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RNF20 (NP_062538.5). |
| Immunogen Sequence: |
MSGIGNKRAAGEPGTSMPPE KKAAVEDSGTTVETIKLGGV SSTEELDIRTLQTKNRKLAE MLDQRQAIEDELREHIEKLE RRQATDDASLLIVNRYWSQF DENIRIILKRYDLEQGLGDL LTERKALVVPEPEPDSDSNQ ERKDDRERGEGQEPAFSFLA TLASSSSEEMESQLQERVES SRRAVSQIVTVYDKLQEKVE LLSRKLNSGDNLIVEEAVQE LNSFLAQENMRLQELTDLL |
| Tissue Specificity | Expressed in the normal brain and also in malignant gliomas (at protein level). |
| Function | Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B.reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Bre1aBre1-AHbre1Ring Finger Protein 20Ring-Type E3 Ubiquitin Transferase Bre1a |
| Database Links | Reactome: R-HSA-8866654Reactome: R-HSA-9013422 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Bre1a antibodyAnti-Bre1-A antibodyAnti-Hbre1 antibodyAnti-Ring Finger Protein 20 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Bre1a antibodyAnti-RNF20 antibodyAnti-BRE1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
