Anti-RNF181 antibody (1-153) (STJ116616)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RNF181 (1-153) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF181 |
| Gene ID | 51255 |
| Uniprot ID | RN181_HUMAN |
| Immunogen Region | 1-153 |
| Immunogen Sequence | MASYFDEHDCEPSDPEQETR TNMLLELARSLFNRMDFEDL GLVVDWDHHLPPPAAKTVVE NLPRTVIRGSQAELKCPVCL LEFEEEETAIEMPCHHLFHS SCILPWLSKTNSCPLCRYEL PTDDDTYEEHRRDKARKQQQ QHRLENLHGAMYT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human RNF181 (NP_057578.1). |
Additional Info
| Tissue Specificity | Widely expressed, with highest levels in liver and heart and lowest levels in brain and skeletal muscle. Expressed in platelets (at protein level). |
| Post Translational Modifications | Autoubiquitinated as part of the enzymatic reaction. |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Catalyzes monoubiquitination of 26S proteasome subunit PSMC2/RPT1. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf181Ring Finger Protein 181 |
| Database Links | Reactome: R-HSA-8866654 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf181 antibodyAnti-Ring Finger Protein 181 antibodyAnti-RNF181 antibodyAnti-HSPC238 antibody |
Information sourced from Uniprot.org