| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RNF181 |
| Gene ID: | 51255 |
| Uniprot ID: | RN181_HUMAN |
| Immunogen Region: | 1-153 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human RNF181 (NP_057578.1). |
| Immunogen Sequence: | MASYFDEHDCEPSDPEQETR TNMLLELARSLFNRMDFEDL GLVVDWDHHLPPPAAKTVVE NLPRTVIRGSQAELKCPVCL LEFEEEETAIEMPCHHLFHS SCILPWLSKTNSCPLCRYEL PTDDDTYEEHRRDKARKQQQ QHRLENLHGAMYT |
| Tissue Specificity | Widely expressed, with highest levels in liver and heart and lowest levels in brain and skeletal muscle. Expressed in platelets (at protein level). |
| Post Translational Modifications | Autoubiquitinated as part of the enzymatic reaction. |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Catalyzes monoubiquitination of 26S proteasome subunit PSMC2/RPT1. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf181Ring Finger Protein 181 |
| Database Links | Reactome: R-HSA-8866654 |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf181 antibodyAnti-Ring Finger Protein 181 antibodyAnti-RNF181 antibodyAnti-HSPC238 antibody |
Information sourced from Uniprot.org