Anti-RNF152 antibody (150-200) (STJA0006445)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human RN152 AA range: 150-200
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase RNF152 (150-200) for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF152 |
| Gene ID | 220441 |
| Uniprot ID | RN152_HUMAN |
| Immunogen | Synthesized peptide derived from human RN152 AA range: 150-200 |
| Immunogen Region | 150-200 |
| Specificity | This antibody detects endogenous levels of human RN152 |
Additional Info
| Post Translational Modifications | Ubiquitinated. Autoubiquitinated in vitro, leading to its degradation by the proteasome (Probable). |
| Function | E3 ubiquitin-protein ligase that acts as a negative regulator of mTORC1 signaling by mediating ubiquitination of RagA/RRAGA and RHEB. Catalyzes 'Lys-63'-linked polyubiquitination of RagA/RRAGA in response to amino acid starvation, thereby regulating mTORC1 signaling. Also mediates monoubiquitination of RHEB, promoting its association with the TSC-TBC complex and subsequent inhibition. Also mediates 'Lys-48'-linked polyubiquitination of target proteins and their subsequent targeting to the proteasome for degradation. Induces apoptosis when overexpressed. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf152Ring Finger Protein 152Ring-Type E3 Ubiquitin Transferase Rnf152 |
| Database Links | Reactome: R-HSA-8866654 |
| Cellular Localisation | Lysosome MembraneSingle-Pass Membrane Protein |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf152 antibodyAnti-Ring Finger Protein 152 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf152 antibodyAnti-RNF152 antibody |
Information sourced from Uniprot.org