• Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RNF139 antibody (10-60 aa) (STJ196033)

SKU:
STJ196033

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase RNF139 (10-60 aa) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNF139
Gene ID: 11236
Uniprot ID: RN139_HUMAN
Immunogen Region: 10-60 aa
Specificity: This antibody detects endogenous levels of RN139 at Human/Mouse
Immunogen: Synthesized peptide derived from the human RN139 at the amino acid range 10-60
Post Translational Modifications Autoubiquitinated. Ubiquitination is induced by sterol and leads to ist degradation via the ubiquitin-proteasome pathway.
Function E3-ubiquitin ligase.acts as a negative regulator of cell proliferation through mechanisms involving G2/M arrest and cell death. Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER). Affects SREBP processing by hindering the SREBP-SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression. Involved in the sterol-accelerated degradation of HMGCR. This is achieved through binding of RNF139 to INSIG1 and/or INSIG2 at the ER membrane. In addition, interaction of RNF139 with AUP1 facilitates interaction of RNF139 with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase AMFR, leading to ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction. Required for INSIG1 ubiquitination. May be required for EIF3 complex ubiquitination.
Protein Name E3 Ubiquitin-Protein Ligase Rnf139
Ring Finger Protein 139
Ring-Type E3 Ubiquitin Transferase Rnf139
Translocation In Renal Carcinoma On Chromosome 8 Protein
Database Links Reactome: R-HSA-901032
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Rnf139 antibody
Anti-Ring Finger Protein 139 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Rnf139 antibody
Anti-Translocation In Renal Carcinoma On Chromosome 8 Protein antibody
Anti-RNF139 antibody
Anti-TRC8 antibody

Information sourced from Uniprot.org

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