Anti-RNF139 antibody (10-60 aa) (STJ196033)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human RN139 at the amino acid range 10-60
General Information
| Short Description | Rabbit polyclonal anti-E3 ubiquitin-protein ligase RNF139 (10-60 aa) for use in WB and IHC in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF139 |
| Gene ID | 11236 |
| Uniprot ID | RN139_HUMAN |
| Immunogen | Synthesized peptide derived from the human RN139 at the amino acid range 10-60 |
| Immunogen Region | 10-60 aa |
| Specificity | This antibody detects endogenous levels of RN139 at Human/Mouse |
Additional Info
| Post Translational Modifications | Autoubiquitinated. Ubiquitination is induced by sterol and leads to ist degradation via the ubiquitin-proteasome pathway. |
| Function | E3-ubiquitin ligase.acts as a negative regulator of cell proliferation through mechanisms involving G2/M arrest and cell death. Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER). Affects SREBP processing by hindering the SREBP-SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression. Involved in the sterol-accelerated degradation of HMGCR. This is achieved through binding of RNF139 to INSIG1 and/or INSIG2 at the ER membrane. In addition, interaction of RNF139 with AUP1 facilitates interaction of RNF139 with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase AMFR, leading to ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction. Required for INSIG1 ubiquitination. May be required for EIF3 complex ubiquitination. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf139Ring Finger Protein 139Ring-Type E3 Ubiquitin Transferase Rnf139Translocation In Renal Carcinoma On Chromosome 8 Protein |
| Database Links | Reactome: R-HSA-901032 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf139 antibodyAnti-Ring Finger Protein 139 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf139 antibodyAnti-Translocation In Renal Carcinoma On Chromosome 8 Protein antibodyAnti-RNF139 antibodyAnti-TRC8 antibody |
Information sourced from Uniprot.org