| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Gene Symbol: |
RNF135 |
| Gene ID: |
84282 |
| Uniprot ID: |
RN135_HUMAN |
| Immunogen Region: |
121-309 aa |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 121-309 of human RNF135 (NP_115698.3). |
| Immunogen Sequence: |
ELQRVAVEKSITEVAQELTE LVEHLVDIVRSLQNQRPLSE SGPDNELSILGKAFSSGVDL SMASPKLVTSDTAAGKIRDI LHDLEEIQEKLQESVTWKEA PEAQMQGELLEAPSSSSCPL PDQSHPALRRASRFAQWAIH PTFNLKSLSCSLEVSKDSRT VTVSHRPQPYRWSCERFSTS QVLCSQALS |
| Tissue Specificity | Expressed in skeletal muscle, spleen, kidney, placenta, prostate, stomach, thyroid and tongue. Also weakly expressed in heart, thymus, liver and lung. |
| Post Translational Modifications | (Microbial infection) Cleaved and inactivated by hepatitis C virus NS3/NS4A. |
| Function | E2-dependent E3 ubiquitin-protein ligase that functions as a RIGI coreceptor in the sensing of viral RNAs in cell cytoplasm and the activation of the antiviral innate immune response. Together with the UBE2D3, UBE2N and UB2V1 E2 ligases, catalyzes the 'Lys-63'-linked polyubiquitination of RIGI oligomerized on viral RNAs, an essential step in the activation of the RIG-I signaling pathway. Through a ubiquitin-independent parallel mechanism, which consists in bridging RIGI filaments forming on longer viral RNAs, further activates the RIG-I signaling pathway. This second mechanism that synergizes with the ubiquitin-dependent one would thereby allow an RNA length-dependent regulation of the RIG-I signaling pathway (Probable). Associated with the E2 ligase UBE2N, also constitutively synthesizes unanchored 'Lys-63'-linked polyubiquitin chains that may also activate the RIG-I signaling pathway. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf135Rig-I E3 Ubiquitin LigaseReulRing Finger Protein 135Ring Finger Protein Leading To Rig-I ActivationRipletRing-Type E3 Ubiquitin Transferase Rnf135 |
| Database Links | Reactome: R-HSA-168928Reactome: R-HSA-5689896Reactome: R-HSA-918233Reactome: R-HSA-933541Reactome: R-HSA-933542Reactome: R-HSA-933543Reactome: R-HSA-936440Reactome: R-HSA-9705671 |
| Cellular Localisation | CytoplasmStress Granule |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf135 antibodyAnti-Rig-I E3 Ubiquitin Ligase antibodyAnti-Reul antibodyAnti-Ring Finger Protein 135 antibodyAnti-Ring Finger Protein Leading To Rig-I Activation antibodyAnti-Riplet antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf135 antibodyAnti-RNF135 antibodyAnti-L13 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
