Anti-RNF125 antibody (1-105) (STJ117360)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RNF125 (1-105) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF125 |
| Gene ID | 54941 |
| Uniprot ID | RN125_HUMAN |
| Immunogen Region | 1-105 |
| Immunogen Sequence | MGSVLSTDSGKSAPASATAR ALERRRDPELPVTSFDCAVC LEVLHQPVRTRCGHVFCRSC IATSLKNNKWTCPYCRAYLP SEGVPATDVAKRMKSEYKNC AECDT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human RNF125 (NP_060301.2). |
Additional Info
| Tissue Specificity | Predominantly expressed in lymphoid tissues, including bone marrow, spleen and thymus. Also weakly expressed in other tissues. Predominant in the CD4(+) and CD8(+) T-cells, suggesting that it is preferentially confined to T-cells. |
| Post Translational Modifications | Autoubiquitinated, leading to its subsequent proteasomal degradation. |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as RIGI, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53. Acts as a negative regulator of type I interferon production by mediating ubiquitination of RIGI at 'Lys-181', leading to RIGI degradation. Mediates ubiquitination and subsequent degradation of p53/TP53. Mediates ubiquitination and subsequent degradation of JAK1. Acts as a positive regulator of T-cell activation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf125Ring Finger Protein 125T-Cell Ring Activation Protein 1Trac-1 |
| Database Links | Reactome: R-HSA-936440 |
| Cellular Localisation | Golgi Apparatus MembraneLipid-AnchorShows A Reticular Staining Pattern Within The Cell And Is Probably Expressed At Other Intracellular Membranes In Addition To The Golgi MembraneNot Detected At The Plasma Membrane |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf125 antibodyAnti-Ring Finger Protein 125 antibodyAnti-T-Cell Ring Activation Protein 1 antibodyAnti-Trac-1 antibodyAnti-RNF125 antibody |
Information sourced from Uniprot.org