• Western blot analysis of extracts of various cell lines, using RIOX2/RIOX2/MINA53 Rabbit monoclonal antibody (STJ11103150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RIOX2/MINA53 antibody (350-450) [S0MR] (STJ11103150)

SKU:
STJ11103150

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RIOX2/MINA53 (350-450) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RIOX2
Gene ID: 84864
Uniprot ID: RIOX2_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human RIOX2/MINA53 (Q8IUF8).
Immunogen Sequence: LSTPGGKLPRLDSVVRLQFK DHIVLTVLPDQDQSDEAQEK MVYIYHSLKNSRETHMMGNE EETEFHGLRFPLSHLDALKQ IWNSPAISVKDLKLTTDEEK E
Tissue Specificity Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
Function Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
Protein Name Ribosomal Oxygenase 2
60s Ribosomal Protein L27a Histidine Hydroxylase
Bifunctional Lysine-Specific Demethylase And Histidyl-Hydroxylase Mina
Histone Lysine Demethylase Mina
Myc-Induced Nuclear Antigen
Mineral Dust-Induced Gene Protein
Nucleolar Protein 52
Ribosomal Oxygenase Mina
Rox
Database Links Reactome: R-HSA-3214842
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Ribosomal Oxygenase 2 antibody
Anti-60s Ribosomal Protein L27a Histidine Hydroxylase antibody
Anti-Bifunctional Lysine-Specific Demethylase And Histidyl-Hydroxylase Mina antibody
Anti-Histone Lysine Demethylase Mina antibody
Anti-Myc-Induced Nuclear Antigen antibody
Anti-Mineral Dust-Induced Gene Protein antibody
Anti-Nucleolar Protein 52 antibody
Anti-Ribosomal Oxygenase Mina antibody
Anti-Rox antibody
Anti-RIOX2 antibody
Anti-MDIG antibody
Anti-MINA antibody
Anti-MINA53 antibody
Anti-NO52 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance