| Host: |
Mouse |
| Applications: |
FC/IF/IHC |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Ribonucleoproteins is suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
58-15 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgM Kappa |
| Formulation: |
PBS with 0.02% Sodium Azide. |
| Purification: |
Affinity purified from tissue culture. |
| Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: |
Flow cytometry (1-2 µg/million cells in 0, 1ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunofluorescence (0, 5-1, 0 µg/ml). Immunohistology (2-4 Mu g/ml for 30 min at RT; staining of |
| Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: |
HNRNPA2B1 |
| Gene ID: |
3181 |
| Uniprot ID: |
ROA2_HUMAN |
| Immunogen: |
A BALB/c mouse was immunized with isolated nuclei. Fusion partner: NS-1. |
| Post Translational Modifications | Sumoylated in exosomes, promoting miRNAs-binding. Asymmetric dimethylation at Arg-266 constitutes the major methylation site. According to a report, methylation affects subcellular location and promotes nuclear localization. According to another report, methylation at Arg-266 does not influence nucleocytoplasmic shuttling. |
| Function | Heterogeneous nuclear ribonucleoprotein (hnRNP) that associates with nascent pre-mRNAs, packaging them into hnRNP particles. The hnRNP particle arrangement on nascent hnRNA is non-random and sequence-dependent and serves to condense and stabilize the transcripts and minimize tangling and knotting. Packaging plays a role in various processes such as transcription, pre-mRNA processing, RNA nuclear export, subcellular location, mRNA translation and stability of mature mRNAs. Forms hnRNP particles with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Involved in transport of specific mRNAs to the cytoplasm in oligodendrocytes and neurons: acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) or the A2RE11 (derivative 11 nucleotide oligonucleotide) sequence motifs present on some mRNAs, and promotes their transport to the cytoplasm. Specifically binds single-stranded telomeric DNA sequences, protecting telomeric DNA repeat against endonuclease digestion. Also binds other RNA molecules, such as primary miRNA (pri-miRNAs): acts as a nuclear 'reader' of the N6-methyladenosine (m6A) mark by specifically recognizing and binding a subset of nuclear m6A-containing pri-miRNAs. Binding to m6A-containing pri-miRNAs promotes pri-miRNA processing by enhancing binding of DGCR8 to pri-miRNA transcripts. Involved in miRNA sorting into exosomes following sumoylation, possibly by binding (m6A)-containing pre-miRNAs. Acts as a regulator of efficiency of mRNA splicing, possibly by binding to m6A-containing pre-mRNAs. Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform. Also plays a role in the activation of the innate immune response. Mechanistically, senses the presence of viral DNA in the nucleus, homodimerizes and is demethylated by JMJD6. In turn, translocates to the cytoplasm where it activates the TBK1-IRF3 pathway, leading to interferon alpha/beta production. (Microbial infection) Involved in the transport of HIV-1 genomic RNA out of the nucleus, to the microtubule organizing center (MTOC), and then from the MTOC to the cytoplasm: acts by specifically recognizing and binding the A2RE (21 nucleotide hnRNP A2 response element) sequence motifs present on HIV-1 genomic RNA, and promotes its transport. |
| Protein Name | Heterogeneous Nuclear Ribonucleoproteins A2/B1Hnrnp A2/B1 |
| Database Links | Reactome: R-HSA-72163Reactome: R-HSA-72203Reactome: R-HSA-8950505 |
| Cellular Localisation | NucleusNucleoplasmCytoplasmCytoplasmic GranuleSecretedExtracellular ExosomeLocalized In Cytoplasmic Mrnp Granules Containing Untranslated MrnasComponent Of RibonucleosomesNot Found In The NucleolusFound In Exosomes Following SumoylationIsoform A2: NucleusPredominantly NucleoplasmicHowever Is Also Found In The Cytoplasm Of Cells In Some Tissues |
| Alternative Antibody Names | Anti-Heterogeneous Nuclear Ribonucleoproteins A2/B1 antibodyAnti-Hnrnp A2/B1 antibodyAnti-HNRNPA2B1 antibodyAnti-HNRPA2B1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
