| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RHOH (1-191) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:10-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RHOH |
| Gene ID: |
399 |
| Uniprot ID: |
RHOH_HUMAN |
| Immunogen Region: |
1-191 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human RHOH (NP_004301.1). |
| Immunogen Sequence: |
MLSSIKCVLVGDSAVGKTSL LVRFTSETFPEAYKPTVYEN TGVDVFMDGIQISLGLWDTA GNDAFRSIRPLSYQQADVVL MCYSVANHNSFLNLKNKWIG EIRSNLPCTPVLVVATQTDQ REMGPHRASCVNAMEGKKLA QDVRAKGYLECSALSNRGVQ QVFECAVRTAVNQARRRNRR RLFSINECKIF |
| Tissue Specificity | Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis. |
| Post Translational Modifications | Phosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development. |
| Function | Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells. Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils. |
| Protein Name | Rho-Related Gtp-Binding Protein RhohGtp-Binding Protein TtfTranslocation Three Four Protein |
| Database Links | Reactome: R-HSA-9013407 |
| Cellular Localisation | CytoplasmCell MembraneLipid-AnchorCytoplasmic SideColocalizes Together With Zap70 In The Immunological Synapse |
| Alternative Antibody Names | Anti-Rho-Related Gtp-Binding Protein Rhoh antibodyAnti-Gtp-Binding Protein Ttf antibodyAnti-Translocation Three Four Protein antibodyAnti-RHOH antibodyAnti-ARHH antibodyAnti-TTF antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
