• Western blot analysis of various lysates using RHOF Rabbit polyclonal antibody (STJ116259) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RHOF antibody (40-120) (STJ116259)

SKU:
STJ116259

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RHOF (40-120) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHOF
Gene ID: 54509
Uniprot ID: RHOF_HUMAN
Immunogen Region: 40-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-120 of human RHOF (NP_061907.2).
Immunogen Sequence: SQGSFPEHYAPSVFEKYTAS VTVGSKEVTLNLYDTAGQED YDRLRPLSYQNTHLVLICYD VMNPTSYDNVLIKWFPEVTH F
Function Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
Protein Name Rho-Related Gtp-Binding Protein Rhof
Rho Family Gtpase Rif
Rho In Filopodia
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-9035034
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Rho-Related Gtp-Binding Protein Rhof antibody
Anti-Rho Family Gtpase Rif antibody
Anti-Rho In Filopodia antibody
Anti-RHOF antibody
Anti-ARHF antibody
Anti-RIF antibody

Information sourced from Uniprot.org

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