| Host: | Rabbit |
| Applications: | IHC/WB |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-RhoB is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | RHOB |
| Gene ID: | 388 |
| Uniprot ID: | RHOB_HUMAN |
| Specificity: | Specific for RhOB. |
| Immunogen: | A synthetic peptide from human Rho-related GTP-binding protein RhoB conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and rat. |
| Function | Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells. |
| Protein Name | Rho-Related Gtp-Binding Protein RhobRho Cdna Clone 6H6 |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-416482Reactome: R-HSA-416572Reactome: R-HSA-5625740Reactome: R-HSA-5625900Reactome: R-HSA-5627117Reactome: R-HSA-5663220Reactome: R-HSA-5666185Reactome: R-HSA-9013026 |
| Cellular Localisation | Late Endosome MembraneLipid-AnchorCell MembraneNucleusCleavage FurrowLate Endosomal Membrane (Geranylgeranylated Form)Plasma Membrane (Farnesylated Form)Also Detected At The Nuclear Margin And In The NucleusTranslocates To The Equatorial Region Before Furrow Formation In A Ect2-Dependent Manner |
| Alternative Antibody Names | Anti-Rho-Related Gtp-Binding Protein Rhob antibodyAnti-Rho Cdna Clone 6 antibodyAnti-H6 antibodyAnti-RHOB antibodyAnti-ARH6 antibodyAnti-ARHB antibody |
Information sourced from Uniprot.org
