• Western blot analysis of extracts of various cell lines, using RGN antibody (STJ25344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RGN antibody (1-299) (STJ25344)

SKU:
STJ25344

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RGN (1-299) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RGN
Gene ID: 9104
Uniprot ID: RGN_HUMAN
Immunogen Region: 1-299
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human RGN (NP_004674.1).
Immunogen Sequence: MSSIKIECVLPENCRCGESP VWEEVSNSLLFVDIPAKKVC RWDSFTKQVQRVTMDAPVSS VALRQSGGYVATIGTKFCAL NWKEQSAVVLATVDNDKKNN RFNDGKVDPAGRYFAGTMAE ETAPAVLERHQGALYSLFPD HHVKKYFDQVDISNGLDWSL DHKIFYYIDSLSYSVDAFDY DLQTGQISNRRSVYKLEKEE QIPDGMCIDAEGKLWVACYN GGRVIRLDPVTGKRLQTVK
Function Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities.
Protein Name Regucalcin
Rc
Gluconolactonase
Gnl
Senescence Marker Protein 30
Smp-30
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Regucalcin antibody
Anti-Rc antibody
Anti-Gluconolactonase antibody
Anti-Gnl antibody
Anti-Senescence Marker Protein 30 antibody
Anti-Smp-30 antibody
Anti-RGN antibody
Anti-SMP30 antibody

Information sourced from Uniprot.org

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