• Western blot analysis of extracts of various cell lines, using RGMA antibody (STJ11102718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-RGMA antibody (350-450) [S8MR] (STJ11102718)

SKU:
STJ11102718

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RGMA (350-450) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RGMA
Gene ID: 56963
Uniprot ID: RGMA_HUMAN
Immunogen Region: 350-450
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 350-450 of human RGMA (Q96B86).
Immunogen Sequence: TAPETFPYETAVAKCKEKLP VEDLYYQACVFDLLTTGDVN FTLAAYYALEDVKMLHSNKD KLHLYERTRDLPGRAAAGLP LAPRPLLGALVPLLALLPVF C
Post Translational Modifications Autocatalytically cleaved at low pH.the two chains remain linked via two disulfide bonds.
Function Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
Protein Name Repulsive Guidance Molecule A
Rgm Domain Family Member A
Database Links Reactome: R-HSA-373752
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Repulsive Guidance Molecule A antibody
Anti-Rgm Domain Family Member A antibody
Anti-RGMA antibody
Anti-RGM antibody

Information sourced from Uniprot.org

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