Anti-RFX3 antibody (280-430) (STJ117506)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RFX3 (280-430) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RFX3 |
| Gene ID | 5991 |
| Uniprot ID | RFX3_HUMAN |
| Immunogen Region | 280-430 |
| Immunogen Sequence | QQKQRYKPMQKVDGVADGFT GSGQQTGTSVEQTVIAQSQH HQQFLDASRALPEFGEVEIS SLPDGTTFEDIKSLQSLYRE HCEAILDVVVNLQFSLIEKL WQTFWRYSPSTPTDGTTITE SSNLSEIESRLPKAKLITLC KHESILKWMCN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 280-430 of human RFX3 (NP_602304.1). |
Additional Info
| Function | Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells. |
| Protein Name | Transcription Factor Rfx3Regulatory Factor X 3 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor Rfx3 antibodyAnti-Regulatory Factor X 3 antibodyAnti-RFX3 antibody |
Information sourced from Uniprot.org