Anti-RFTN1 antibody (432-481 aa) (STJ95354)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RFTN1 at the amino acid range 432-481
General Information
| Short Description | Rabbit polyclonal anti-Raftlin (432-481 aa) for use in WB and IHC in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RFTN1 |
| Gene ID | 23180 |
| Uniprot ID | RFTN1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RFTN1 at the amino acid range 432-481 |
| Immunogen Region | 432-481 aa |
| Specificity | Raftlin Polyclonal Antibody detects endogenous levels of Raftlin protein. |
Additional Info
| Function | Involved in protein trafficking via association with clathrin and AP2 complex. Upon bacterial lipopolysaccharide stimulation, mediates internalization of TLR4 to endosomes in dendritic cells and macrophages.and internalization of poly(I:C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells.resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production. Involved in T-cell antigen receptor-mediated signaling by regulating tyrosine kinase LCK localization, T-cell dependent antibody production and cytokine secretion. May regulate B-cell antigen receptor-mediated signaling. May play a pivotal role in the formation and/or maintenance of lipid rafts. |
| Protein Name | RaftlinCell Migration-Inducing Gene 2 ProteinRaft-Linking Protein |
| Database Links | |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmMembrane RaftEndosomeEarly EndosomeTranslocates From Cytoplasm To Cell Membrane Where It Colocalizes With Poly (I:c) And Then Moves To Endosomes Where It Colocalizes With Tlr3Translocates From Cytoplasm To Cell Membrane Where It Colocalizes With Tlr4 And Then Together With Tlr4 Moves To EndosomesUpon Lipopolysaccharide Stimulation |
| Alternative Antibody Names | Anti-Raftlin antibodyAnti-Cell Migration-Inducing Gene 2 Protein antibodyAnti-Raft-Linking Protein antibodyAnti-RFTN1 antibodyAnti-KIAA0084 antibodyAnti-MIG2 antibody |
Information sourced from Uniprot.org