Anti-RFTN1 antibody (1-260) (STJ112462)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RFTN1 (1-260) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RFTN1 |
| Gene ID | 23180 |
| Uniprot ID | RFTN1_HUMAN |
| Immunogen Region | 1-260 |
| Immunogen Sequence | MGCGLNKLEKRDEKRPGNIY STLKRPQVETKIDVSYEYRF LEFTTLSAAELPGSSAVRLA SLRDLPAQLLELYQQGFSLA ALHPFVQPTHEREKTPLEHI FRAILIKKTDRSQKTDLHNE GYILELDCCSSLDHPTDQKL IPEFIKKIQEAASQGLKFVG VIPQYHSSVNSAGSSAPVST ANSTEDARDAKNARGDHASL ENEKPGTGDVCSAPAGRNQS PEPSSGPRGEVPLAKQPSS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RFTN1 (NP_055965.1). |
Additional Info
| Tissue Specificity | Expressed in B-cells (at protein level). Expressed in dendritic cells and macrophages. |
| Function | Involved in protein trafficking via association with clathrin and AP2 complex. Upon bacterial lipopolysaccharide stimulation, mediates internalization of TLR4 to endosomes in dendritic cells and macrophages.and internalization of poly(I:C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells.resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production. Involved in T-cell antigen receptor-mediated signaling by regulating tyrosine kinase LCK localization, T-cell dependent antibody production and cytokine secretion. May regulate B-cell antigen receptor-mediated signaling. May play a pivotal role in the formation and/or maintenance of lipid rafts. |
| Protein Name | RaftlinCell Migration-Inducing Gene 2 ProteinRaft-Linking Protein |
| Database Links | |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmMembrane RaftEndosomeEarly EndosomeTranslocates From Cytoplasm To Cell Membrane Where It Colocalizes With Poly (I:c) And Then Moves To Endosomes Where It Colocalizes With Tlr3Translocates From Cytoplasm To Cell Membrane Where It Colocalizes With Tlr4 And Then Together With Tlr4 Moves To EndosomesUpon Lipopolysaccharide Stimulation |
| Alternative Antibody Names | Anti-Raftlin antibodyAnti-Cell Migration-Inducing Gene 2 Protein antibodyAnti-Raft-Linking Protein antibodyAnti-RFTN1 antibodyAnti-KIAA0084 antibodyAnti-MIG2 antibody |
Information sourced from Uniprot.org