• Anti-RFP antibody at 1:2000 dilution; 293HEK cells transduced with RFP Ad; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunofluorescence – anti-RFP antibody in 293HEK cells transfected with RFP at 1:50 dilution; cells were fixed with 4% of PFA
  • Immunogold labeling of epithelium cells, in vivo injected with RFP expressing vector

Anti-RFP antibody (STJ140119)

SKU:
STJ140119

Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF/ELISA
Reactivity: Transfected cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Red Fluorescent Protein is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:500-1:2000
IHC-F 1:50-1:500
IHC-P 1:50-1:500
IF 1:50-1:500
ELISA ND
Tan Z Kong M Wen S et al. J Bone Miner Res 2020 Jul. PMID: 32662900
Endo T Kadoya K Suzuki Y et al. Front Cell Neurosci 2019 Jun. PMID: 31316351
Jian
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).
Immunogen: Purified recombinant RFP peptide produced in E. coli.
Background Goat polyclonal antibody to RFP (Red fluorescent protein). RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance