| Short Description : | Rabbit polyclonal anti-Riboflavin kinase (82-132 aa) for use in WB, ELISA and IHC in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA/IHC |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RFK |
| Gene ID: | 55312 |
| Uniprot ID: | RIFK_HUMAN |
| Immunogen: | Synthesized peptide derived from the human RIFK at the amino acid range 82-132 |
| Immunogen Region: | 82-132 aa |
| Specificity: | This antibody detects endogenous levels of RIFK at Human/Mouse |
| Function | Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase. |
| Protein Name | Riboflavin KinaseAtp -Riboflavin 5'-PhosphotransferaseFlavokinase |
| Database Links | Reactome: R-HSA-196843 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Riboflavin Kinase antibodyAnti-Atp -Riboflavin 5'-Phosphotransferase antibodyAnti-Flavokinase antibodyAnti-RFK antibody |
Information sourced from Uniprot.org
