Anti-RFK antibody (1-155) (STJ116344)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RFK (1-155) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RFK |
| Gene ID | 55312 |
| Uniprot ID | RIFK_HUMAN |
| Immunogen Region | 1-155 |
| Immunogen Sequence | MRHLPYFCRGQVVRGFGRGS KQLGIPTANFPEQVVDNLPA DISTGIYYGWASVGSGDVHK MVVSIGWNPYYKNTKKSMET HIMHTFKEDFYGEILNVAIV GYLRPEKNFDSLESLISAIQ GDIEEAKKRLELPEHLKIKE DNFFQVSKSKIMNGH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-155 of human RFK (NP_060809.3). |
Additional Info
| Tissue Specificity | Detected in brain, placenta and urinary bladder. |
| Function | Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase. |
| Protein Name | Riboflavin KinaseAtp -Riboflavin 5'-PhosphotransferaseFlavokinase |
| Database Links | Reactome: R-HSA-196843 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Riboflavin Kinase antibodyAnti-Atp -Riboflavin 5'-Phosphotransferase antibodyAnti-Flavokinase antibodyAnti-RFK antibody |
Information sourced from Uniprot.org