• Western blot analysis of extracts of various cell lines, using RFK antibody (STJ116344) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using RFK Rabbit polyclonal antibody (STJ116344) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RFK antibody (1-155) (STJ116344)

SKU:
STJ116344

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RFK (1-155) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RFK
Gene ID: 55312
Uniprot ID: RIFK_HUMAN
Immunogen Region: 1-155
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-155 of human RFK (NP_060809.3).
Immunogen Sequence: MRHLPYFCRGQVVRGFGRGS KQLGIPTANFPEQVVDNLPA DISTGIYYGWASVGSGDVHK MVVSIGWNPYYKNTKKSMET HIMHTFKEDFYGEILNVAIV GYLRPEKNFDSLESLISAIQ GDIEEAKKRLELPEHLKIKE DNFFQVSKSKIMNGH
Function Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.
Protein Name Riboflavin Kinase
Atp -Riboflavin 5'-Phosphotransferase
Flavokinase
Database Links Reactome: R-HSA-196843
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Riboflavin Kinase antibody
Anti-Atp -Riboflavin 5'-Phosphotransferase antibody
Anti-Flavokinase antibody
Anti-RFK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance