• Biotinylated STJ71977 (1ug/ml) staining of Human Breast Cancer lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-Restin/CLIP1, Biotinylated antibody (C-Term) {Biotin} (STJ73493)

SKU:
STJ73493-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Restin/CLIP1, Biotinylated (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CLIP1
Gene ID: 6249
Uniprot ID: CLIP1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_002947.1; NP_937883.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_002947.1; NP_937883.1).
Immunogen Sequence: PHSTHHGSRGEERP.
Post Translational Modifications Phosphorylated. Phosphorylation induces conformational changes by increasing the affinity of the N-terminus for C-terminus, resulting in inhibition of its function thus decreasing its binding to microtubules and DCTN1. Exhibits a folded, autoinhibited conformation when phosphorylated and an open conformation when dephosphorylated with increased binding affinity to microtubules and DCTN1. Phosphorylation regulates its recruitment to tyrosinated microtubules and the recruitment of vesicular cargo to microtubules in neurons. Phosphorylation by MTOR may positively regulate CLIP1 association with microtubules.
Function Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.
Protein Name Cap-Gly Domain-Containing Linker Protein 1
Cytoplasmic Linker Protein 1
Cytoplasmic Linker Protein 170 Alpha-2
Clip-170
Reed-Sternberg Intermediate Filament-Associated Protein
Restin
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5626467
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Cytoskeleton
Cytoplasmic Vesicle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Projection
Ruffle
Localizes To Microtubule Plus Ends
Localizes Preferentially To The Ends Of Tyrosinated Microtubules
Accumulates In Plasma Membrane Regions With Ruffling And Protrusions
Associates With The Membranes Of Intermediate Macropinocytic Vesicles
Alternative Antibody Names Anti-Cap-Gly Domain-Containing Linker Protein 1 antibody
Anti-Cytoplasmic Linker Protein 1 antibody
Anti-Cytoplasmic Linker Protein 170 Alpha-2 antibody
Anti-Clip-170 antibody
Anti-Reed-Sternberg Intermediate Filament-Associated Protein antibody
Anti-Restin antibody
Anti-CLIP1 antibody
Anti-CYLN1 antibody
Anti-RSN antibody

Information sourced from Uniprot.org

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