Anti-Repulsive guidance molecule A antibody [R54-5O-5] (STJA0035530)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human RGMA
General Information
| Short Description | Rabbit monoclonal anti-Repulsive guidance molecule A for use in WB, ICC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R54-5O-5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200 |
| Formulation | Liquid in PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | RGMA |
| Gene ID | 56963 |
| Uniprot ID | RGMA_HUMAN |
| Immunogen | A synthesized peptide derived from human RGMA |
Additional Info
| Post Translational Modifications | Autocatalytically cleaved at low pH.the two chains remain linked via two disulfide bonds. |
| Function | Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. |
| Protein Name | Repulsive Guidance Molecule ARgm Domain Family Member A |
| Database Links | Reactome: R-HSA-373752 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-Anchor |
| Alternative Antibody Names | Anti-Repulsive Guidance Molecule A antibodyAnti-Rgm Domain Family Member A antibodyAnti-RGMA antibodyAnti-RGM antibody |
Information sourced from Uniprot.org