| Host: |
Rabbit |
| Applications: |
WB/ELISA/IHC |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ATP-dependent DNA helicase Q5 (647-697 aa) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RECQL5 |
| Gene ID: |
9400 |
| Uniprot ID: |
RECQ5_HUMAN |
| Immunogen Region: |
647-697 aa |
| Specificity: |
This antibody detects endogenous levels of RECQ5 at Human |
| Immunogen: |
Synthesized peptide derived from the human RECQ5 at the amino acid range 647-697 |
| Post Translational Modifications | Phosphorylated by CDK1 at Ser-727.this phosphorylation is required for RECQL5-mediated disruption of RAD51 filaments on stalled replication forks. |
| Function | DNA helicase that plays an important role in DNA replication, transcription and repair. Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability. Associates also with POLR1A and enforces the stability of ribosomal DNA arrays. Plays an important role in mitotic chromosome separation after cross-over events and cell cycle progress. Mechanistically, removes RAD51 filaments protecting stalled replication forks at common fragile sites and stimulates MUS81-EME1 endonuclease leading to mitotic DNA synthesis. Required for efficient DNA repair, including repair of inter-strand cross-links. Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination. |
| Protein Name | Atp-Dependent Dna Helicase Q5Dna Helicase - Recq-Like Type 5Recq5Recq Protein-Like 5 |
| Cellular Localisation | Isoform Beta: NucleusNucleoplasmNucleusRecruited To Sites Of Dna DamageSuch As Single-Strand Breaks And Inter-Strand Cross-LinksAnd At Stalled Replication ForksRe-Localizes From The Nucleolus Into The Nucleus After Replicative Stress And Significantly Associates With Wrn During S-PhaseIsoform Alpha: CytoplasmIsoform Gamma: Cytoplasm |
| Alternative Antibody Names | Anti-Atp-Dependent Dna Helicase Q5 antibodyAnti-Dna Helicase - Recq-Like Type 5 antibodyAnti-Recq5 antibodyAnti-Recq Protein-Like 5 antibodyAnti-RECQL5 antibodyAnti-RECQ5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
