Anti-RECQL5 antibody (647-697 aa) (STJ195797)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human RECQ5 at the amino acid range 647-697
General Information
| Short Description | Rabbit polyclonal anti-ATP-dependent DNA helicase Q5 (647-697 aa) for use in WB, ELISA and IHC in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RECQL5 |
| Gene ID | 9400 |
| Uniprot ID | RECQ5_HUMAN |
| Immunogen | Synthesized peptide derived from the human RECQ5 at the amino acid range 647-697 |
| Immunogen Region | 647-697 aa |
| Specificity | This antibody detects endogenous levels of RECQ5 at Human |
Additional Info
| Post Translational Modifications | Phosphorylated by CDK1 at Ser-727.this phosphorylation is required for RECQL5-mediated disruption of RAD51 filaments on stalled replication forks. |
| Function | DNA helicase that plays an important role in DNA replication, transcription and repair. Probably unwinds DNA in a 3'-5' direction (Probable). Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability. Also associates with POLR1A and enforces the stability of ribosomal DNA arrays. Plays an important role in mitotic chromosome separation after cross-over events and cell cycle progress. Mechanistically, removes RAD51 filaments protecting stalled replication forks at common fragile sites and stimulates MUS81-EME1 endonuclease leading to mitotic DNA synthesis. Required for efficient DNA repair, including repair of inter-strand cross-links. Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination. A core helicase fragment (residues 11-609) binds preferentially to splayed duplex, looped and ssDNA. |
| Protein Name | Atp-Dependent Dna Helicase Q5Dna 3'-5' Helicase Recq5Dna Helicase - Recq-Like Type 5Recq5Recq Protein-Like 5 |
| Database Links | |
| Cellular Localisation | Isoform Beta: NucleusNucleoplasmNucleusRecruited To Sites Of Dna DamageSuch As Single-Strand Breaks And Inter-Strand Cross-LinksAnd At Stalled Replication ForksRe-Localizes From The Nucleolus Into The Nucleus After Replicative Stress And Significantly Associates With Wrn During S-PhaseIsoform Alpha: CytoplasmIsoform Gamma: Cytoplasm |
| Alternative Antibody Names | Anti-Atp-Dependent Dna Helicase Q5 antibodyAnti-Dna 3'-5' Helicase Recq5 antibodyAnti-Dna Helicase - Recq-Like Type 5 antibodyAnti-Recq5 antibodyAnti-Recq Protein-Like 5 antibodyAnti-RECQL5 antibodyAnti-RECQ5 antibody |
Information sourced from Uniprot.org