Anti-Recombinant-ZO1 tight junction protein antibody [SR0620] (STJA0010620)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein corresponding to Mouse ZO-1
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-ZO1 tight junction protein for use in IHC and IF in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SR0620 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | IHC/IF (H, M, R) 1:400-1:1200 |
| Formulation | PBS with 0.15% ProClin300, 100 Mu g/mL BSA and 50% glycerol. |
| Storage Instruction | Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
Target Information
| Gene Symbol | TJP1 |
| Gene ID | 7082 |
| Uniprot ID | ZO1_HUMAN |
| Immunogen | Recombinant protein corresponding to Mouse ZO-1 |
Additional Info
| Post Translational Modifications | Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ. |
| Function | TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus. May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions. |
| Protein Name | Tight Junction Protein 1Tight Junction Protein Zo-1Zona Occludens Protein 1Zonula Occludens Protein 1 |
| Database Links | Reactome: R-HSA-191650Reactome: R-HSA-2028269Reactome: R-HSA-351906Reactome: R-HSA-8935964Reactome: R-HSA-9705677 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell JunctionTight JunctionGap JunctionCell ProjectionPodosomeMoves From The Cytoplasm To The Cell Membrane Concurrently With Cell-Cell ContactForms A Condensed Tight Junction-Linked Belt Of Protein During Junction Formation Which Becomes Anchored To The Apical Cell Membrane Via Interaction With PatjAt Podosomal SitesIs Predominantly Localized In The Ring Structure Surrounding The Actin CoreColocalizes With Spef1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells |
| Alternative Antibody Names | Anti-Tight Junction Protein 1 antibodyAnti-Tight Junction Protein Zo-1 antibodyAnti-Zona Occludens Protein 1 antibodyAnti-Zonula Occludens Protein 1 antibodyAnti-TJP1 antibodyAnti-ZO1 antibody |
Information sourced from Uniprot.org