Anti-Recombinant-VAV3 antibody [RM2K84] (STJA0025949)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Guanine Nucleotide Exchange Factor Vav3 for use in FC, IF, IP and WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IF/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2K84 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | FCM: 1:20-1:100, IF: 1:50-1:200, IP: 1:20-1:50, WB: 1:1000-1:2000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | VAV3 |
| Gene ID | 10451 |
| Uniprot ID | VAV3_HUMAN |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylation can be mediated by ROS1. In osteoclasts, undergoes tyrosine phosphorylation in response to CSF1. |
| Function | Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion. |
| Protein Name | Guanine Nucleotide Exchange Factor Vav3Vav-3 |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-193648Reactome: R-HSA-2029482Reactome: R-HSA-2424491Reactome: R-HSA-2871796Reactome: R-HSA-2871809Reactome: R-HSA-3928665Reactome: R-HSA-416482Reactome: R-HSA-4420097Reactome: R-HSA-5218920Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013408Reactome: R-HSA-9664422Reactome: R-HSA-9748787 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Guanine Nucleotide Exchange Factor Vav3 antibodyAnti-Vav-3 antibodyAnti-VAV3 antibody |
Information sourced from Uniprot.org