Anti-Recombinant-VAPA antibody [RM2K54] (STJA0025919)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJA0025919
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General Information

Short DescriptionRabbit monoclonal anti-Recombinant-Vesicle-Associated Membrane Protein-Associated Protein A for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC/WB
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDRM2K54
IsotypeIgG
ConjugationUnconjugated
PurificationProtein A/G purified from cell culture supernatant
Dilution RangeIHC: 1:100-1:200, WB: 1:1000-1:2000
Formulation0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide
Storage InstructionSuitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months.

Target Information

Gene SymbolVAPA
Gene ID9218
Uniprot IDVAPA_HUMAN

Additional Info

Function Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. STARD3-VAPA interaction enables cholesterol transfer from the ER to endosomes. Via interaction with WDR44 participates in neosynthesized protein export. In addition, recruited to the plasma membrane through OSBPL3 binding. The OSBPL3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With OSBPL3, may regulate ER morphology. May play a role in vesicle trafficking.
Protein Name Vesicle-Associated Membrane Protein-Associated Protein A
Vamp-A
Vamp-Associated Protein A
Vap-A
33 Kda Vamp-Associated Protein
Vap-33
Database Links Reactome: R-HSA-1660661
Reactome: R-HSA-6798695
Reactome: R-HSA-9609523
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iv Membrane Protein
Cell Membrane
Cell Junction
Tight Junction
Nucleus Membrane
Present In The Plasma Membrane And In Intracellular Vesicles
Together With Snare Proteins
May Also Associate With The Cytoskeleton
Colocalizes With Ocln At The Tight Junction In Polarized Epithelial Cells
Alternative Antibody Names Anti-Vesicle-Associated Membrane Protein-Associated Protein A antibody
Anti-Vamp-A antibody
Anti-Vamp-Associated Protein A antibody
Anti-Vap-A antibody
Anti-33 Kda Vamp-Associated Protein antibody
Anti-Vap-33 antibody
Anti-VAPA antibody
Anti-VAP33 antibody

Information sourced from Uniprot.org

Citations

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