Anti-Recombinant-VAChT antibody (55-125 aa) [N425/45] (STJA0040481)
SPECIFICATIONS
ClonalityMonoclonal
HostChicken
ConjugationUnconjugated
IsotypeIgY
ImmunogenFusion protein amino acids 55-125 (first vesicle lumen domain) of rat VAChT (accession number Q62666) produced recombinantly in E. Coli
General Information
| Short Description | Chicken monoclonal anti-Recombinant-VAChT (55-125 aa) for use in WB, IHC, ICC and ELISA in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/ICC/ELISA |
| Host | Chicken |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | N425/45 |
| Isotype | IgY |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Purified by affinity chromatography |
| Formulation | PBS |
| Storage Instruction | Aliquot and store at โค-20ยฐC for long term storage. For short term storage, store at 2-8ยฐC. For maximum recovery of product, centrifuge the vial prior to removing the cap. |
Target Information
| Gene Symbol | Slc18a3 |
| Gene ID | 60422 |
| Uniprot ID | VACHT_RAT |
| Immunogen | Fusion protein amino acids 55-125 (first vesicle lumen domain) of rat VAChT (accession number Q62666) produced recombinantly in E. Coli |
| Immunogen Region | 55-125 aa |
| Immunogen Sequence | Rat |
| Specificity | Does not cross-react with VMAT1 or VMAT2 |
Additional Info
| Tissue Specificity | High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus. |
| Function | Electrogenic antiporter that exchanges one cholinergic neurotransmitter, acetylcholine or choline, with two intravesicular protons across the membrane of synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to store neurotransmitters inside the vesicles prior to their release via exocytosis. Determines cholinergic vesicular quantal size at presynaptic nerve terminals in developing neuro-muscular junctions with an impact on motor neuron differentiation and innervation pattern. Part of forebrain cholinergic system, regulates hippocampal synapse transmissions that underlie spatial memory formation. Can transport serotonin. |
| Protein Name | Vesicular Acetylcholine TransporterVachtRvatSolute Carrier Family 18 Member 3 |
| Database Links | Reactome: R-RNO-264642Reactome: -RNO-8856825Reactome: -RNO-8856828 |
| Cellular Localisation | Cytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Vesicular Acetylcholine Transporter antibodyAnti-Vacht antibodyAnti-Rvat antibodyAnti-Solute Carrier Family 18 Member 3 antibodyAnti-Slc18a3 antibodyAnti-Vacht antibodyAnti-Vat antibody |
Information sourced from Uniprot.org