Anti-Recombinant-Tyrosine Hydroxylase antibody [LNC1] (STJA0040571)
SPECIFICATIONS
ClonalityMonoclonal
HostChicken
ConjugationUnconjugated
IsotypeIgY
ImmunogenTyrosine Hydroxylase purified from PC12 cells
General Information
| Short Description | Chicken monoclonal anti-Recombinant-Tyrosine Hydroxylase for use in WB, IHC, ICC, IP and ELISA in Chicken, Frog, Human, Lizard, Monkey, Mouse, Rat, Vole and Zebrafish samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/ICC/IP/ELISA |
| Host | Chicken |
| Reactivity | Chicken/Frog/Human/Lizard/Monkey/Mouse/Rat/Vole/Zebrafish |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | LNC1 |
| Isotype | IgY |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Purified by affinity chromatography |
| Formulation | PBS |
| Storage Instruction | Aliquot and store at โค-20ยฐC for long term storage. For short term storage, store at 2-8ยฐC. For maximum recovery of product, centrifuge the vial prior to removing the cap. |
Target Information
| Gene Symbol | TH |
| Gene ID | 7054 |
| Uniprot ID | TY3H_HUMAN |
| Immunogen | Tyrosine Hydroxylase purified from PC12 cells |
| Immunogen Sequence | Human |
| Specificity | Recognizes an epitope outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: purified dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan |
Additional Info
| Tissue Specificity | Mainly expressed in the brain and adrenal glands. |
| Post Translational Modifications | Phosphorylated on Ser-19, Ser-62 and Ser-71 by several protein kinases with different site specificities. Phosphorylation at Ser-62 and Ser-71 leads to an increase of TH activity. Phosphorylation at Ser-71 activates the enzyme and also counteracts the feedback inhibition of TH by catecholamines. Phosphorylation of Ser-19 and Ser-62 triggers the proteasomal degradation of TH through the ubiquitin-proteasome pathway. Phosphorylation at Ser-62 facilitates transport of TH from the soma to the nerve terminals via the microtubule network. Phosphorylation at Ser-19 induces the high-affinity binding to the 14-3-3 protein YWHAG.this interaction may influence the phosphorylation and dephosphorylation of other sites. Ser-19 increases the phosphorylation at Ser-71 in a hierarchical manner, leading to increased activity. |
| Function | Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa. In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity. Positively regulates the regression of retinal hyaloid vessels during postnatal development. Isoform 5: Lacks catalytic activity. Isoform 6: Lacks catalytic activity. |
| Protein Name | Tyrosine 3-MonooxygenaseTyrosine 3-HydroxylaseTh |
| Database Links | Reactome: R-HSA-209905 |
| Cellular Localisation | CytoplasmPerinuclear RegionNucleusCell ProjectionAxonCytoplasmic VesicleSecretory VesicleSynaptic VesicleWhen Phosphorylated At Ser-19 Shows A Nuclear Distribution And When Phosphorylated At Ser-31 As Well At Ser-40 Shows A Cytosolic DistributionExpressed In Dopaminergic Axons And Axon Terminals |
| Alternative Antibody Names | Anti-Tyrosine 3-Monooxygenase antibodyAnti-Tyrosine 3-Hydroxylase antibodyAnti-Th antibodyAnti-TH antibodyAnti-TYH antibody |
Information sourced from Uniprot.org