Anti-Recombinant-THOC1 antibody [RM2P35] (STJA0026296)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Tho Complex Subunit 1 for use in IF and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2P35 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IF: 1:50-1:200, WB: 1:500-1:1000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | THOC1 |
| Gene ID | 9984 |
| Uniprot ID | THOC1_HUMAN |
Additional Info
| Post Translational Modifications | Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass. Polyubiquitinated, leading to proteasomal degradation.probably involves NEDD4. |
| Function | Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. Required for efficient export of polyadenylated RNA. The THOC1-THOC2-THOC3 core complex alone is sufficient to bind export factor NXF1-NXT1 and promote ATPase activity of DDX39B/UAP56. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation. May play a role in hair cell formation, hence may be involved in hearing. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1. (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. |
| Protein Name | Tho Complex Subunit 1Nuclear Matrix Protein P84P84n5Htrex84 |
| Database Links | Reactome: R-HSA-159236Reactome: R-HSA-72187Reactome: R-HSA-73856 |
| Cellular Localisation | Isoform 1: Nucleus SpeckleNucleusNucleoplasmNucleus MatrixCytoplasmCan Shuttle Between The Nucleus And CytoplasmNuclear Localization Is Required For Induction Of Apoptotic Cell DeathTranslocates To The Cytoplasm During The Early Phase Of Apoptosis ExecutionIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Tho Complex Subunit 1 antibodyAnti-Nuclear Matrix Protein P84 antibodyAnti-P84n5 antibodyAnti-Htrex84 antibodyAnti-THOC1 antibodyAnti-HPR1 antibody |
Information sourced from Uniprot.org