Anti-Recombinant-PRAME antibody [RM2B29] (STJA0025102)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Melanoma antigen Preferentially Expressed In Tumors for use in IHC, IP and WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2B29 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IHC: 1:50-1:100, IP: 1:20, WB: 1:500-1:1000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | PRAME |
| Gene ID | 23532 |
| Uniprot ID | PRAME_HUMAN |
Additional Info
| Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation. The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding. In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation. Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG: prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. |
| Protein Name | Melanoma Antigen Preferentially Expressed In TumorsOpa-Interacting Protein 4Oip-4Preferentially Expressed Antigen Of Melanoma |
| Database Links | |
| Cellular Localisation | NucleusChromosomeCytoplasmGolgi ApparatusCell MembraneAssociates With ChromatinSpecifically Enriched At Transcriptionally Active Promoters That Are Also Bound By Nuclear Transcription Factor Y (Composed Of NfyaNfyb And Nfyc) And At EnhancersRecruited To The Golgi Apparatus In Response To Interferon Gamma (Ifng) Treatment |
| Alternative Antibody Names | Anti-Melanoma Antigen Preferentially Expressed In Tumors antibodyAnti-Opa-Interacting Protein 4 antibodyAnti-Oip-4 antibodyAnti-Preferentially Expressed Antigen Of Melanoma antibodyAnti-PRAME antibodyAnti-MAPE antibodyAnti-OIP4 antibody |
Information sourced from Uniprot.org