Anti-Recombinant-OPN/Spp1 antibody [RM1M67] (STJA0023754)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Osteopontin for use in IHC and WB in Human, Rat and Hamster samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Rat/Hamster |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM1M67 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IHC: 1:50-1:100 |
| Formulation | 0.01M PBS |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | SPP1 |
| Gene ID | 6696 |
| Uniprot ID | OSTP_HUMAN |
Additional Info
| Post Translational Modifications | Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. |
| Function | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Protein Name | OsteopontinBone Sialoprotein 1NephropontinSecreted Phosphoprotein 1Spp-1Urinary Stone ProteinUropontin |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-186797Reactome: R-HSA-216083Reactome: R-HSA-381426Reactome: R-HSA-8949275Reactome: R-HSA-8957275Reactome: R-HSA-9856532 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Osteopontin antibodyAnti-Bone Sialoprotein 1 antibodyAnti-Nephropontin antibodyAnti-Secreted Phosphoprotein 1 antibodyAnti-Spp-1 antibodyAnti-Urinary Stone Protein antibodyAnti-Uropontin antibodyAnti-SPP1 antibodyAnti-BNSP antibodyAnti-OPN antibodyAnti-PSEC0156 antibody |
Information sourced from Uniprot.org