Anti-Recombinant-NOTCH4 antibody [RM2J80] (STJA0025846)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Neurogenic Locus Notch Homolog Protein 4 for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2J80 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | WB: 1:500-1:1000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | NOTCH4 |
| Gene ID | 4855 |
| Uniprot ID | NOTC4_HUMAN |
Additional Info
| Post Translational Modifications | Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. |
| Function | Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. |
| Protein Name | Neurogenic Locus Notch Homolog Protein 4Notch 4Hnotch4 Cleaved Into - Notch 4 Extracellular Truncation - Notch 4 Intracellular Domain |
| Database Links | Reactome: R-HSA-1912399Reactome: R-HSA-1912408Reactome: R-HSA-1912420Reactome: R-HSA-350054Reactome: R-HSA-5083630Reactome: R-HSA-9013695Reactome: R-HSA-9013700Reactome: R-HSA-9604323 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinNotch 4 Intracellular Domain: NucleusFollowing Proteolytical Processing Nicd Is Translocated To The Nucleus |
| Alternative Antibody Names | Anti-Neurogenic Locus Notch Homolog Protein 4 antibodyAnti-Notch 4 antibodyAnti-Hnotch4 Cleaved Into - Notch 4 Extracellular Truncation - Notch 4 Intracellular Domain antibodyAnti-NOTCH4 antibodyAnti-INT3 antibody |
Information sourced from Uniprot.org