| Function | Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis. |
| Protein Name | Dna Mismatch Repair Protein Mlh1Mutl Protein Homolog 1 |
| Database Links | Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5545483Reactome: R-HSA-5632987Reactome: R-HSA-6796648Reactome: R-HSA-912446 |
| Cellular Localisation | NucleusChromosomeRecruited To Chromatin In A Mcm9-Dependent Manner |
| Alternative Antibody Names | Anti-Dna Mismatch Repair Protein Mlh1 antibodyAnti-Mutl Protein Homolog 1 antibodyAnti-MLH1 antibodyAnti-COCA2 antibody |
Information sourced from Uniprot.org
![Anti-MLH1 antibody [SM5040] (STJA0005040)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/180098/403367/STJA0005040_1__67066.1738950433.png?c=1 "Anti-MLH1 antibody [SM5040] (STJA0005040)")
![Western blot analysis of lysates from wild type (WT) and MLH1 knockout (KO) HeLa cells, using [KO Validated] MLH1 Mouse monoclonal antibody (STJ11104591) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/177235/368843/STJ11104591_1__81003.1713134305.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MLH1 knockout (KO) HeLa cells, using [KO Validated] MLH1 Mouse monoclonal antibody (STJ11104591) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")