Anti-Recombinant-MBD2 antibody [RM2K58] (STJA0025923)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-Methyl-Cpg-Binding Domain Protein 2 for use in FC, IF, IHC, IP and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/IF/IHC/IP/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM2K58 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | FCM: 1:20-1:100, IF: 1:50-1:200, IHC: 1:100-1:200, IP: 1:20-1:50, WB: 1:1000-1:5000 |
| Formulation | 0.01M PBS, pH 7.4, 0.05% BSA, 50% Glycerol, 0.05% Sodium Azide |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | MBD2 |
| Gene ID | 8932 |
| Uniprot ID | MBD2_HUMAN |
Additional Info
| Function | Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases to chromatin. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Acts as a transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters. |
| Protein Name | Methyl-Cpg-Binding Domain Protein 2DemethylaseDmtaseMethyl-Cpg-Binding Protein Mbd2 |
| Database Links | Reactome: R-HSA-427413Reactome: R-HSA-73728 |
| Cellular Localisation | NucleusChromosomeNuclearIn Discrete FociDetected At Replication Foci In Late S PhaseLocalizes To Methylated ChromatinLocalizes To Sites Of Dna Damage In A Manner Partially Dependent On Zmynd8 |
| Alternative Antibody Names | Anti-Methyl-Cpg-Binding Domain Protein 2 antibodyAnti-Demethylase antibodyAnti-Dmtase antibodyAnti-Methyl-Cpg-Binding Protein Mbd2 antibodyAnti-MBD2 antibody |
Information sourced from Uniprot.org