Anti-Recombinant-HSD3B1 antibody [RM1P20] (STJA0023905)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal anti-Recombinant-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 for use in IF and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | RM1P20 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A/G purified from cell culture supernatant |
| Dilution Range | IF: 1:50-1:200 |
| Formulation | 0.01M PBS |
| Storage Instruction | Suitable for storage at +4°C between 1-2 weeks. For longer term store at-20°C for up to 12 months. |
Target Information
| Gene Symbol | HSD3B1 |
| Gene ID | 3283 |
| Uniprot ID | 3BHS1_HUMAN |
Additional Info
| Function | A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable). |
| Protein Name | 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 13 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I3-Beta-Hsd I3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase3-Beta-Hydroxysteroid 3-DehydrogenaseDelta-5-3-Ketosteroid IsomeraseDihydrotestosterone OxidoreductaseSteroid Delta-IsomeraseTrophoblast Antigen Fdo161g |
| Database Links | Reactome: R-HSA-193048Reactome: R-HSA-193993Reactome: R-HSA-194002 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMitochondrion Membrane |
| Alternative Antibody Names | Anti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 antibodyAnti-3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I antibodyAnti-3-Beta-Hsd I antibodyAnti-3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase antibodyAnti-3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase antibodyAnti-3-Beta-Hydroxysteroid 3-Dehydrogenase antibodyAnti-Delta-5-3-Ketosteroid Isomerase antibodyAnti-Dihydrotestosterone Oxidoreductase antibodyAnti-Steroid Delta-Isomerase antibodyAnti-Trophoblast Antigen Fdo161g antibodyAnti-HSD3B1 antibodyAnti-3BH antibodyAnti-HSDB3A antibody |
Information sourced from Uniprot.org